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Article info. : 2006; 3(3) (pp 277~284)
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A New Spectrophotometric Method for the Determination of
Nadolol
O.A. Adegoke*, O.S. Idowu and A.A. Olaniyi
Department of Pharmaceutical Chemistry, Faculty of
Pharmacy, University of Ibadan, Ibadan, Nigeria
(Received 27 January 2006, Accepted 12 June 2006)
A new
spectrophotometric method has been developed for the assay of nadolol in
pure form and in tablets. The assay procedure is based on a derivatization
methodology employing 4-carboxyl-2,6-dinitrobenzene diazonium ion (CDNBD) as
a diazo coupling reagent. The azo dye formed between nadolol and CDNBD
absorbed visible light at the wavelength maximum of 416 nm (λmax)
demonstrating a bathochromic shift from the absorption maximum of nadolol.
Optimization studies established an optimal reaction time of 10 min at 60
oC. The assays were linear over 1.25-10 �g ml-1 of
nadolol, and the reaction occurred by a 3:1 reagent/drug stoichiometric
ratio. The method is found to be selective and has a lower detection limit
of 0.29 �g ml-1. Recovery studies over three days gave mean
recovery of 101.4% (RSD 3.0%). This new method has been successfully applied
in the determination of nadolol and nadolol/bendroflumethiazide tablets with
accuracy and precision similar to the official (USP) HPLC procedure (p >
0.05). The new procedure has the advantages of high sensitivity, lower limit
of detection and could find application as an in-process quality control
method for nadolol.
Keywords:
Nadolol, Diazo coupling, Phenol ether, CDNBD, Spectrophotometry |
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