Current location: JICS Archive > Vol. 7 > Supplement. 1 > Articles : 7
Sensitive Spectrophotometric Detection of Dopamine, Levodopa and Adrenaline Using Surface Plasmon Resonance Band of Silver Nanoparticles
M.R. Hormozi Nezhada,b,*, J. Tashkhourianc and J. Khodaveisic
aDepartment of Chemistry, Sharif University of Technology, Tehran, 11155-9516, Iran
b Institute for Nanoscience and Nanotechnology, Sharif University of Technology,
Tehran, P.O. Box 11365-9161, Iran
c Department of Chemistry, Faculty of Sciences, Persian Gulf University, Bushehr 75169, Iran
A simple and effective procedure is proposed for spectrophotometric determination of catecholamines; Dopamine (1), L-Dopa (2) and Adrenaline (3). It was found that the reduction of Ag+ to silver nanoparticles (Ag-NPs) by these catecholamines in the presence of polyvinylpyrrolidone (PVP) as a stabilizing agent produced very intense surface plasmon resonance peak of Ag-NPs. The plasmon absorbance of the Ag-NPs allows the quantitative spectrophotometric detection of the catecholamines. The calibration curves derived from the changes in absorbance at λ = 440 nm were linear with concentration of Dopamine, Levodopa and Adrenaline in the range of 3.2×10-6- 2.0×10-5 M, 1.6×10-7 - 1.0×10-5 M, 1.5×10-6- 4.0×10-5 M, respectively. The detection limits (3σ) were 1.2×10-6 M, 8.6 ×10-8 M, 9.7 ×10-7 M for the Dopamine, L-Dopa and Adrenaline, respectively. The method was applied successfully to the determination of catecholamines in Ringer’s injection serum.
Keywords: Silver nanoparticles, Catecholamines, Surface plasmon band, Spectrophotometric determination
